Table 5.

(a–e). Multiple comparison test of LSmeans of nutrient quantity (g ha⁻¹) in partitioned plant tissues at various stages comparing treatment effects with control.

(a)
Location	Component and Stages	Control	T1R1	T1R2	T2R1	T2R2	T1R1 & T2R1	T3R1	T3R2	T2R1 & T3R1
B	Leaf
	V6	2.7(0.5) †, ‡	2.2 ‡	2.2 ‡	2.5 ‡	2.7 ‡	1.8 ‡	2.8 ‡	3.3 ‡	3.3 ‡
	V13	18.5(2.3) ‡	21.0 §	21.7 §	16.9 ‡	19.6 ‡	22.2+ §	15.4 ‡	18.8 ‡	18.4 ‡
	V17	31.7(6.3) ‡	32.9	34.9	50.3 ** §	48.5 ** §	51.1 ** §	31.3 ‡	31.3 ‡	45.5 * §
	R2	24.1(2.1) ‡	25.3	27.0	28.9+	26.9	29.0+	30.0* §	38.9 *** §	28.9+ §
	R6	17.5(2.1) ‡	15.1	16.8	21.3+	15.1	18.2	17.2	18.7	17.0
	Stalk
	V6	1.8(0.3) ‡	1.4 ‡	1.7 ‡	1.6 ‡	1.5 ‡	1.6 ‡	2.0 ‡	1.4 ‡	1.5 ‡
	V13	14.0(3.8) ‡	17.1 §	13.1 §	11.2 ‡	13.0 ‡	19.8+ §	12.5 ‡	14.0 ‡	11.7 ‡
	V17	20.9(4.9) ‡	26.1	28.8 +	27.3 §	30.9** §	34.6** §	24.1 ‡	26.0 ‡	28.9 + §
	R2	26.9(3.0) ‡	39.5**	31.4	34.8 +	32.1	42.2**	34.1 + §	36.0* §	32.5 §
	R6	5.3(4.7) ‡	5.4	5.8	11.9	5.1	6.3	5.3	12.6 +	6.1
	Reproductive
	R2	15.2(3.1) ‡	16.4	18.6	16.3	16.3	15.2	17.7 §	18.6 §	22.9 + §
	R6	6.5(2.9) ‡	16.8***	5.9	8.1	7.5	8.4	7.8	9.9	7.0
	Total
	V6	4.5(0.5) ‡	3.6 ‡	3.9 ‡	4.1 ‡	4.1 ‡	3.4 ‡	4.7 ‡	4.7 ‡	4.8 ‡
	V13	32.5(5.0) ‡	38.1 §	34.9 §	28.1 ‡	32.6 ‡	42.0 + §	27.9 ‡	32.8 ‡	30.1 ‡
	V17	52.6(8.2) ‡	59.0	63.7	77.5 ** §	79.4** §	85.7 *** §	55.4 ‡	57.3 ‡	74.4 ** §
	R2	66.2(5.0) ‡	81.2*	77.0	80 +	75.4	86.4 **	81.7* §	93.5 *** §	84.2 * §
	R6	29.3(7.0) ‡	37.3	28.5	41.3 +	27.8	32.9	30.3	41.2 +	30.0
(b)
Location	Component and stages	Control	T1R1	T1R2	T2R1	T2R2	T1R1 & T2R1	T3R1	T3R2	T2R1 & T3R1
Mn	Leaf
	V7	56.7(7.3) ‡	59.2 ‡	58.4 ‡	66.2 ‡	58.1 ‡	58.0 ‡	52.6 ‡	58.4 ‡	73.4 ‡
	V15	125.9(32.7) ‡	225.1 ** §	272.4 *** §	167.3 ‡	135.1 ‡	202.5* §	149.3 ‡	145.5 ‡	162.7 ‡
	VT	147.0(24.6) ‡	189.9 +	227.1 **	213.1 ** §,¶	216.8 ** §	237.6*** §	146.1 ‡	151.1 ‡	210.2 ** §
	R3	219.8(25.0) ‡	251.1	412.5 ***	248.3	288.1 +	335.1**	287.3 + §	326.2** §	346.1 *** §
	R6	295.4(30.9) ‡	344.6 +	442.44 ***	347.4 +	380.1**	416.8***	346.7 +	428.0***	403.4***
	Stalk
	V7	27.3(5.8) ‡	35.4 ‡	38.5 ‡	40.8 ‡	35.8 ‡	35.7 ‡	39.4 ‡	30.1 ‡	38.9 ‡
	V15	197.0(28.1) ‡	251.6 + §	249.0 + §	206.4 ‡	189.7 ‡	225.8 §	178.8 ‡	168.0 ‡	202.1 ‡
	VT	259.4(37.3) ‡	305.7	267.9	339.1* §,¶	293.3 §	325.8 + §	280.1 ‡	264.4 ‡	303.7 §
	R3	257.3(26.4) ‡	320.8 +	353.8**	303.1	359.8**	414.0 ***	329.2 + §	406.4 *** §	377.3 ** §
	R6	118.5(23.2) ‡	97.4	133.5	84.2	123.9	163.1 +	115.7	141.5	134.9
	Reproductive
	VT	5.3(1.4) ‡	6.9	6.2	8.7 ** §,¶	6.4 §	6.5 §	5.7 ‡	7.2 ‡	7.6 + §
	R3	79.3(12.8) ‡	75.8	87.1	88.6	79.0	92.8	98.2 §	103.8 §	92.5 §
	R6	31.1(11.6) ‡	42.1	39.0	35.8	29.3	40.6	45.9	47.8	53.6 +
	Grain
	R3	14.4(1.7) ‡	13.0	13.2	16.5	12.9	18.0	15.5 §	11.7 §	14.7 §
	R6	72.4(6.5) ‡	85.1 +	76.2	83.8 +	77.7	84.8 +	81.3	85.4*	78.0
	Total
	V6	84.0(11.6) ‡	94.6 ‡	96.9 ‡	107.0 ‡	93.9 ‡	93.7 ‡	92.0 ‡	88.5 ‡	112.3 + ‡
	V13	322.9(47.8) ‡	476.7 ** §	521.4 *** §	373.6 ‡	324.8 ‡	428.3 ** §	328.1 ‡	313.5 ‡	364.8 ‡
	V17	411.7(57.0) ‡	502.5	501.0	561.0** §	516.4 + §	569.9 ** §	432.0 ‡	422.7 ‡	521.6 + §
	R2	570.8(52.4) ‡	660.8	845.2***	681.0	739.7*	857.6 ***	730.2 * §	850.6 *** §	830.6*** §
	R6	517.4(52.9) ‡	569.1	691.2**	551.2	611.0 +	705.2 ***	589.5	702.7 ***	669.9 **
(c)
Location	Component and Stages	Control	T1R1	T1R2	T2R1	T2R2	T1R1 & T2R1	T3R1	T3R2	T2R1 & T3R1
Zn only	Leaf
	V6	10.5(0.7) ‡	11.1 ‡	9.9 ‡	9.5 ‡	11.0 ‡	10.4 ‡	10.9 ‡	11.7 ‡	12.7* ‡
	V14	51.9(13.9) ‡	154.5 *** §	213.6 *** §	45.9 ‡	47.2 ‡	123.9*** §,#	53.1 ‡	45.5 ‡	47.6 ‡
	V17	86.6(26.8) ‡	175.0**	227.9 ***	207.4 *** §	298.6 *** §	242.0 *** §,#	75.9 ‡	77.5 ‡	206.8*** §
	R2	74.6(52.9) ‡	125.0	192.3 *	135.5	174.8 +	292.4 ***	152.5 §	209.6* §	213.3** §
	R6	102.1(23.7) ‡	153.1 *	210.9 ***	169.7**	216.1 ***	200.5***	148.5 +	224.3***	213.1 ***
	Stalk
	V6	10.5(1.3) ‡	10.3 ‡	7.3 ‡	8.9 ‡	11.9 ‡	10.2 ‡	11.6 ‡	10.3 ‡	13.8 + ‡
	V14	94.9(36.4) ‡	135.2 §	210.7 ** §	117.9 ‡	98.6 ‡	134.2 §,#	78.0 ‡	89.8 ‡	96.6 ‡
	V17	101.0(15.6) ‡	123.8	192.2 ***	148.0 ** §	244.9 *** §	178.5 *** §,#	109.5 ‡	100.7 ‡	151.0 ** §
	R2	115.0(25.2) ‡	150.3	181.1**	159.0 +	213.8***	200.9**	147.6 §	256.8*** §	234.9*** §
	R6	140.1(36.0) ‡	137.9	136.5	145.4	197.3	164.2	155.5	214.5*	193.8
	Reproductive
	R2	54.6(6.1) ‡	57.1	61.3	58.4	64.2	60.7	70.9** §	91.1*** §	80.4*** §
	R6	82.6(15.0) ‡	81.4	108.9 +	71.2	78.2	75.5	72.6	89.8	87.6
	Grain
	R2	20.0(2.0) ‡	17.7	15.2*	15.3*	17.2	17.6	16.2 + §	20.0 §	20.1 §
	R6	243.2(12.4) ‡	252.3	242.0	232.9	229.1	230.9	265.5 +	246.7	249.2
	Total
	V6	21.1(2.2) ‡	21.4 ‡	14.9* ‡	18.4 ‡	22.9 ‡	20.6 ‡	24.5 ‡	22.0 ‡	26.5* ‡
	V14	146.8(44.5) ‡	289.7* §	424.3 *** §	163.8 ‡	145.9 ‡	258.1** §	131.1 ‡	135.3 ‡	144.1 ‡
	V17	187.7(32.6) ‡	298.8**	420.1 ***	355.7 *** §	543.5 *** §	420.6*** §	185.4 ‡	178.2 ‡	357.9 *** §
	R2	264.1(60.6) ‡	350.2	449.9 *	368.3 +	470.0 **	571.5 ***	387.2* §	577.6 *** §	548.7 *** §
	R6	568.0(51.9) ‡	624.6	698.2 *	619.2	720.7 **	671.2 *	642.1	775.2 ***	743.7 **
(d)
Location	Component and Stages	Control	T1R1	T1R2	T2R1	T2R2	T1R1 & T2R1	T3R1	T3R2	T4R1
Fe/Zn (Zn Values)	Leaf
	V6	12.0(0.9) ‡	10.0 + ‡	10.9 ‡	12.2 ‡	15.1** ‡	13.7 ‡	11.8 ‡	11.5 ‡	11.9 ‡
	V14	40.6(25.0) ‡	138.0** §	93.4 §	96.0 + ‡	72.5 ‡	124.9* §	55.6 ‡	44.9 ‡	49.1 ‡
	V17	75.3(14.0) ‡	145.3***	149.9***	123.2* §	189.3*** §	186.0*** §	53.2 ‡	66.5 ‡	55.3 ‡
	R2	73.9(17.0) ‡	118.4 +	184.4***	103.6	158.8***	149.7**	82.7 §	139.3** §	64.8 ‡
	R6	64.3(6.8) ‡	66.8	104.9***	66.7	95.1**	100.9***	63.2	77.0	62.7 §
	Stalk
	V6	13.7(1.8) ‡	8.8 + ‡	11.0 ‡	10.7 ‡	18.4 + ‡	11.9 ‡	8.6* ‡	11.0 ‡	11.9 ‡
	V14	67.2(8.5) ‡	90.4 + §	87.2 + §	97.5* ‡	66.7 ‡	93.4* §	64.9 ‡	58.5 ‡	71.4 ‡
	V17	119.0(14.7) ‡	116.3	154.8 +	124.2 §	140.01 §	132.3 §	102.6 ‡	103.6 ‡	86.7 + ‡
	R2	102.0(13.5) ‡	85.7	107.9	91.9	121.0	115.0	109.2 §	107.7 §	79.1 ‡
	R6	94.7(14.6) ‡	65.6	73.5	70.4	48.6*	74.8	77.9	45.5*	59.4 + §
	Reproductive
	R2	65.8(5.7) ‡	64.9	66.0	74.9	70.0	72.1	74.3 §	72.0 §	72.3 ‡
	R6	54.4(6.7) ‡	56.4	63.8	56.6	65.7	77.3*	58.1	69.7 +	53.9 §
	Grain
	R2	20.0(2.0) ‡	18.7	19.3	17.3	18.9	22.5	30.0 ** §	20.0 §	14.7 + ‡
	R6	210.0(14.3) ‡	206.9	213.4	194.7	212.3	241.6 +	209.6	220.4	206.5 §
	Total
	V6	25.8(2.4) ‡	18.8* ‡	22.7 ‡	22.9 ‡	33.6 * ‡	24.9 ‡	20.4 ‡	22.5 ‡	23.8 ‡
	V14	107.7(28.5) ‡	228.5** §	180.6 + §	193.5* ‡	139.2 ‡	218.3 ** §	120.5 ‡	103.4 ‡	120.5 ‡
	V17	194.3(23.6) ‡	261.5*	304.8**	247.4 §	329.4 *** §	318.4*** §	155.8 ‡	170.1 ‡	141.9 ‡
	R2	261.7(25.0) ‡	287.8	377.6**	287.7	372.5 **	359.3 **	307.4 §	339.2* §	230.8 ‡
	R6	423.5(31.2) ‡	395.7	455.5	388.4	421.7	494.6	408.7	416.1	382.5 §
(e)
Location	Component and Stages	Control	T1R1	T1R2	T2R1	T2R2	T1R1 & T2R1	T3R1	T3R2	T4R1
Fe/Zn (Fe Values)	Leaf
	V6	144.9(12.1) ‡	110.2* ‡	121.6 ‡	124.4 ‡	170.4 ‡	145.8 ‡	130.1 ‡	136.8 ‡	130.8 ‡
	V14	247.7(32.9) ‡	311.8 §	248.0 §	294.3 ‡	276.4 ‡	315.0 ‡ §	235.4 ‡	259.5 ‡	281.4 ‡
	V17	351.0(32.1) ‡	380.6	374.3	339.7 §	434.7 + §	426.9 + §	266.3 + ‡	289.7 ‡	293.8 ‡
	R2	724.9(123.3) ‡	589.3	686.2	528.7	763.9	542.7	391.9** §	547.3 §	575.7 ‡
	R6	213.0(18.2) ‡	196.0	224.9	196.5	227.6	227.0	171.3 +	207.6	175.8 §
	Stalk
	V6	47.2(7.0) ‡	37.7 ‡	35.5 ‡	35.3 ‡	46.8 ‡	44.6 ‡	29.8 + ‡	41.8 ‡	44.7 ‡
	V14	64.8(12.6) ‡	61.6 §	65.2 §	69.7 ‡	86.0 ‡	85.9 §	66.5 ‡	87.8 ‡	71.6 ‡
	V17	167.8(51.5) ‡	141.8	131.0	129 §	228.5 §	152.1 §	154.7 ‡	287.7 + ‡	155.6 ‡
	R2	436.0(63.1) ‡	230.5*	251.6*	208.1 *	372.4	341.7	330.2 §	260.6 + §	269.7 + ‡
	R6	116.1(24.8) ‡	125.0	130.8	108.0	114.4	164.8	171.9 +	169.2 +	170.9 + §
	Reproductive
	R2	160.5(33.0) ‡	222.5	163.5	126.1	128.8	147.5	143.1 §	103.9 §	124.7 ‡
	R6	66.2(17.1) ‡	60.4	54.8	62.1	70.1	101.6	62.3	76.8	48.6 §
	Grain
	R2	14.2(1.9) ‡	12.4	13.6	11.6	15.7	15.6	18.4 §	12.9 §	10.0 ‡
	R6	203.7(15.2) ‡	196.2	235.4	193.7	208.4	221.7	205.7	212.5	190.4 + §
	Total
	V6	192.1(15.0) ‡	147.9* ‡	157.2 ‡	159.7 ‡	217.1 ‡	189.5 ‡	159.9 ‡	178.6 ‡	175.5 ‡
	V14	312.5(35.8) ‡	373.4 §	313.2 §	364 ‡	362.4 ‡	400.9 + §	301.9 ‡	347.3 ‡	353.0 ‡
	V17	518.8(69.0) ‡	522.4	505.3	468.8 §	663.2 §	579.0 §	421.0 ‡	577.4 ‡	449.3 ‡
	R2	1335.6(38.2) ‡	1054.7	1114.9	874.5*	1190.4	1047.5	923.5 + §	924.7* §	980.1 + ‡
	R6	599.1(32.7) ‡	577.5	645.8	560.6	620.5	715.2 *	611.2	630.5	585.7 §

† Least square mean plant nutrient quantity (g ha-1) followed by (SE for all values in the same row) and significant F test: Not Significant >0.10; + >0.05; * > 0.01; ** > 0.001; *** < 0.001. ‡ No foliar treatment had been applied at this stage. § First sampling following foliar treatment. ¶ First sampling following foliar treatment for plots with significant increase on grain yield. # First sampling following foliar treatment for plots with significant decrease on grain yield.