Table 4.
qPCR Determination of Relative Gene Expression (Ct) and “Fold Difference” * in Larger Groups.
| Genes Downregulated in the TM Group Relative to the Control Group | ||||||||
|---|---|---|---|---|---|---|---|---|
| Gene | Group | Mean Ct | Std. Dev. | N | df | F | p-Value | “Fold Difference” * |
| AHSP | Control TM |
32.5668 34.5163 |
2.1561 1.4674 |
22 23 |
1 | 12.674 | 0.001 | 3.86 |
| ALOX12 | Control TM |
30.6055 31.1267 |
0.7140 1.1756 |
22 23 |
1 | 3.194 | 0.081 | 1.44 |
| CD22 | Control TM |
30.7102 31.0753 |
0.8499 1.1344 |
22 23 |
1 | 1.482 | 0.230 | 1.29 |
| ITGB3 | Control TM |
26.4452 27.0511 |
0.7939 1.1830 |
22 23 |
1 | 4.031 | 0.051 | 1.52 |
| ITGB5 | Control TM |
30.9789 31.4723 |
0.6233 0.7281 |
22 23 |
1 | 5.938 | 0.019 | 1.41 |
| LMNA | Control TM |
29.8050 30.2638 |
0.7014 0.9137 |
22 23 |
1 | 3.546 | 0.066 | 1.37 |
| MYL9 | Control TM |
27.1693 27.5611 |
0.6926 1.1940 |
22 23 |
1 | 1.792 | 0.188 | 1.31 |
| SOCS3 | Control TM |
31.4033 32.5206 |
0.9121 1.3372 |
22 23 |
1 | 10.626 | 0.002 | 2.17 |
| TAL1 | Control TM |
30.7461 31.1823 |
0.6561 0.8969 |
22 23 |
1 | 3.441 | 0.070 | 1.35 |
| Genes Upregulated in the TM Group Relative to the Control Group | ||||||||
| CDKN1C | Control TM |
30.2448 29.9343 |
0.7156 0.7260 |
22 23 |
1 | 2.085 | 0.156 | 1.24 |
| CXCL10 | Control TM |
32.7365 31.7125 |
0.9466 0.9613 |
22 23 |
1 | 12.950 | 0.001 | 2.03 |
| HES4 | Control TM |
34.1132 34.3267 |
0.6820 0.7179 |
22 23 |
1 | 1.044 | 0.313 | 1.16 |
| IGFBP7 | Control TM |
29.8499 29.5057 |
0.8188 0.8956 |
22 23 |
1 | 1.805 | 0.186 | 1.27 |
| IL1B | Control TM |
31.4125 31.0576 |
1.0132 0.8328 |
22 23 |
1 | 1.655 | 0.205 | 1.28 |
| TLR4 | Control TM |
29.0808 28.8832 |
0.7732 0.7260 |
22 23 |
1 | 0.782 | 0.381 | 1.15 |
* “Fold Difference” = 2 (∆∆Ct); Ct = threshold cycle.