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Description of gating process. (A) Gating of lymphocytes and identification of (B) CD3+, (C) CD3+CD4+, (D) CD3+CD8+, (E) CD3–CD(16+56)+ cells, CD28– cells on CD4+ population, (F) CD4+CD28null cells and CD28– cells in CD8+ population, (G) CD8+CD28null cells.
