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. 2021 Mar 6;9(3):230. doi: 10.3390/vaccines9030230

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Cellular and innate antiviral immune responses 10 weeks after immunization (wpi). Cellular responses CD8α, IFNγ and Granzyme A (A) and innate antiviral responses viperin, myxovirus resistance gene (Mx) and interferon-stimulated gene (ISG)15 (B) were analyzed by RT-qPCR in spleen, normalized for the reference gene EF1α and shown as 2^−ΔΔCt levels. Gene expression in spleen samples from fish injected with PRV-1 (red), PRV-2 (blue), PRV-3 (green), inactivated PRV (yellow) and mock lysate (gray) are shown.