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. 2021 Mar 6;9(3):230. doi: 10.3390/vaccines9030230

Figure 6.

Figure 6

Histopathology and scores of A. salmon hearts. The status of Atlantic salmon hearts was scored for heart and skeletal muscle inflammation (HSMI) pathology 18 weeks after immunization and eight weeks after PRV-1 cohabitant challenge. The scoring of pancarditis was performed according to a visual analogue scale from 0 to 3, where 0 represents a healthy heart, scores above 1 represent hearts with increased cellularity due to immune cell recruitment in the outer epicardial layer, and more severe cases (a score of 2,5) also show increased cellularity in the outer compact and inner spongious layers of the heart ventricle. (A) PRV-1 ip injected Week 0, (B) PRV-2 immunized ip + PRV-1, (C) PRV-3 immunized ip + PRV-1, (D) inactivated InPRV-1 immunized ip + PRV-1, (E) negative control, mock-injected ip, (F) positive control, mock-injected ip + PRV-1, and (G) a table and violin plot showing pathology scores of individual fish in each experimental group (n = 8) pre-injected with PRV-1 (red), PRV-2 (Blue), PRV-3 (green), Inactivated PRV-1 (Yellow), or mock (grey), then secondary infected with PRV-1 where marked.