Figure 15.

(A) Apoptosis induction by ZVS-08 and compounds 1 and 8 (50 µM) on tumour spheroids formed by Colo 357 cells. ZVS-08 was markedly faster and compound 8 was slowest. Data represent mean ± standard error (N = 3). (B) Representative images of spheroids 24 h after the addition of the indicated compounds (50 µM). Fluorescence intensity in the upper image corresponds to cleavage of the caspase 3/7 activity reporter; it has been merged with the phase contrast image in the lower raw. Scale bar: 400 µm.