Figure 1.

Inhibition of Symbiodiniaceae cell acquisition by carbohydrates or anti-ActL antibody. Juvenile Acropora tenuis polyps were incubated at 25 °C with inhibitors for 1 h. Then, polyps were incubated with Symbiodiniaceae NBRC102920 cells for 6 h. The number of Symbiodiniaceae cells in juvenile A. tenuis polyps was counted using in vivo chlorophyll a fluorescence. (a) Juvenile polyps were incubated with sugars. GlcNAc, NANA, and Suc indicate N-acetyl-d-glucosamine, N-acetyl-d-neuramic acid, and sucrose, respectively. Control indicates no carbohydrate addition. Values are the mean ± SD (n = 4). (b) Juvenile polyps were incubated without antibodies, with normal rabbit IgG, or with anti-ActL antibody. Values are the mean ± SD (n = 12). Different letters indicate significant differences between antibody treatments (p < 0.05, Tukey’s multiple comparisons test).