Figure 2.

Examination of the occurrence of ActL in rearing seawater of juvenile A. tenuis by dot blotting assay. Samples were spotted on the PVDF membrane and detected using the anti-ActL antibody. Samples were 0.1 mg of bovine serum albumin (BSA), 0.1 mg of ActL, and artificial seawater (ASW), where 10 juvenile polyps were kept for 1 d. Binding of the anti-ActL antibody to purified ActL and rearing ASW of juvenile A. tenuis polyps was examined. (a) Each sample solution dot blotted onto a PVDF membrane. BSA was used as a negative control. Purified ActL was used as a positive control. (b) Anti-ActL antibody was preincubated with ActL, and the mixture was used for dot blotting assay instead of the purified anti-ActL antibody.