Figure 3.
The chemotactic activity of various Symbiodiniaceae strains. (a) A capillary containing ActL was placed in NBRC102920 culture in a 35 mm dish. The movements of the Symbiodiniaceae cells were video-recorded. White circle indicates the insertion point of the capillary, red indicates Symbiodiniaceae cells, and cyan lines indicate traces of Symbiodiniaceae cells. (b) The chemotactic assay was conducted for 1 h using Symbiodiniaceae cells at a concentration of 1 × 106 cells/mL. One unit (U) of chemotactic activity was defined as the activity at which 40 Symbiodiniaceae cells were attracted by sample solutions in 60 min. Values are the mean ± SD (n = 3). Different letters indicate a significant difference in chemotactic activity (p < 0.05, Tukey’s multiple comparisons test). The letters in parentheses indicate genera of Symbiodiniaceae; A = genus Symbiodinium, B = genus Breviolum, D = genus Durusdinium, E = genus Effrenium, and F = genus Fugacium.