Figure 5.

Effects of diaportheone A1 (A) and diaportheone A2 (B) on H₂O₂-induced intracellular ROS accumulation. The level of ROS in neuroblastoma SH-SY5Y cells was evaluated using 2′,7′-dichlorodihydrofluorescein diacetate (H₂DCFDA) reagent. SH-SY5Y cells were pretreated with the compounds for 2 h, followed by treatment with 100 μM H₂O₂ for 4 h. No Oxidative Stress indicates treatment of the SH-SY5Y cells only with the compounds. The intracellular ROS level (% of the control cells) was expressed as the mean ± SEM (n = 3). The (*) represents statistical difference (p < 0.05) of the % ROS versus the H₂O₂-treated alone group using one-way ANOVA followed by Tukey’s test.