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. 2021 Mar 13;22(6):2925. doi: 10.3390/ijms22062925

Figure 4.

Figure 4

Influence of pre-/diabetic conditions on viability of THP-1 cells and SaOS-2 cells in 2D and 3D cocultures. THP-1 cells and SaOS-2 cells (2:1 ratio) were cocultured over 14 days in both (A,C) 2D and (B,D) 3D on pHEMA-based cryogel scaffolds in the presence or absence of high insulin levels (HI = prediabetic conditions) or high glucose levels (HG = diabetic conditions). On days 1, 4, 7, 10, and 14 of culture, (A,B) mitochondrial activity (Resazurin conversion) and (C,D) cell-specific DNA content were determined. To consider the entire differentiation period, data are presented as area under the curve (AUC/mean ± SEM). Mitochondrial activities were compared by nonparametric Friedmann test, followed by Dunn’s multiple comparison test. Cell-specific DNA contents were compared by nonparametric two-way ANOVA, followed by Tukey’s multiple comparison test. * p < 0.05, ** p < 0.01, and *** p < 0.0001 as indicated. N = 3, n = 3.