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. 2021 Mar 12;13(6):1261. doi: 10.3390/cancers13061261

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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TGF-β signaling modulates CHPF-mediated malignant phenotypes. (A) Hepa1-6 and HA59T transfectants were starved for 3 h and then treated with (+) or without (−) TGF-β (20 ng/mL) for 15 and 30 min. Cell lysates (20 μg) were analyzed by Western blotting with various antibodies, as indicated. (B) Effects of LY364947, a TGF-β inhibitor, on CHPF-mediated cell viability. HA59T transfectants were treated with a solvent (0.02% DMSO) or LY364947 (0.5 μM) and then analyzed by CCK8 assay. * p < 0.05, ** p < 0.01. (C) Effects of LY364947 on CHPF-mediated migration (left) and invasion (right). HA59T transfectants were treated with a solvent (0.1% DMSO) or LY364947 (2.5 μM) and then analyzed by transwell migration and invasion assays. The data are shown as the means ± standard deviations. ** p < 0.01.