Figure 4.

Real-time RT-qPCR quantification of (A) Tnfα mRNA and (B) Il-1β mRNA in bone marrow-derived macrophages. Cells were incubated with nanospheres for 24 h. Results were compared with untreated cells (control), cells treated with lipopolysaccharide in concentration 100 ng/mL (LPS) to stimulate proinflammatory cytokines expression, dexamethasone solution in a concentration of 200 nM/mL (DEX) and RhB solution in concentration 15 µg/mL (RhB). Data are presented as a fold change in expression relative to untreated control cells (set to be 1). Figure shows a representative experiment data. * p < 0.05, ** p < 0.01, *** p < 0.001.