Table 1.
Characteristics of PLGA nanospheres used in the study prepared by the nanoprecipitation (NPM) and the emulsification solvent evaporation (ESE) methods.
| Batch No. | Polymer | Preparation Method | Fluorescent Tracer | Size (nm) ± SD | PDI ± SD | Zeta Potential (mV) ± SD |
|---|---|---|---|---|---|---|
| 1a | PLGA 50:50 | NPM | - | 157.5 ± 3.7 | 0.06 ± 0.02 | −27 ± 3 |
| 1b | PLGA 50:50 | NPM | RhB | 171.5 ± 1.4 | 0.10 ± 0.01 | −25 ± 2 |
| 1c | PLGA 50:50 | ESE | - | 91.1 ± 1.2 | 0.09 ± 0.01 | −22 ± 3 |
| 1d | PLGA 50:50 | ESE | RhB | 91.2 ± 5.1 | 0.16 ± 0.03 | −22 ± 1 |
| 2a | PLGA 70:30 | NPM | - | 138.0 ± 6.5 | 0.07 ± 0.03 | −28 ± 2 |
| 2b | PLGA 70:30 | NPM | RhB | 166.7 ± 1.6 | 0.08 ± 0.01 | −29 ± 3 |
| 2c | PLGA 70:30 | ESE | - | 81.4 ± 1.8 | 0.08 ± 0.01 | −24 ± 2 |
| 2d | PLGA 70:30 | ESE | RhB | 81.1 ± 7.9 | 0.07 ± 0.01 | −22 ± 1 |
| 3a | Branched PLGA | NPM | - | 131 ± 6.0 | 0.08 ± 0.01 | −32 ± 2 |
| 3b | Branched PLGA | NPM | RhB | 162.0 ± 9.0 | 0.08 ± 0.01 | −33 ± 1 |
| 3c | Branched PLGA | ESE | - | 97.7 ± 5.7 | 0.10 ± 0.01 | −27 ± 2 |
| 3d | Branched PLGA | ESE | RhB | 89.3 ± 8.6 | 0.08 ± 0.03 | −24 ± 1 |
| 4a | Purasorb 5002 | NPM | - | 145.3 ± 0.8 | 0.1 ± 0.01 | −24 ± 3 |
| 4b | Purasorb 5002 | NPM | RhB | 164.6 ± 5.5 | 0.1 ± 0.01 | −24 ± 1 |
RhB-model substance Rhodamine B; PDI-polydispersity index. Data represent the mean ± standard deviation (SD) of three measurements.