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. 2021 Mar 16;10(3):663. doi: 10.3390/cells10030663

Figure 3.

Figure 3

Piezo1-induced stiffening does not require Piezo1 channel activity at the plasma membrane, calpain activity, integrin-ß1 and ROCK, but FAK in HEK cells. Average stiffness of cells: 2 days after wash-out of antibiotics (AB, 172 µM streptomycin and 200 µM penicillin, (A)); after DMSO treatment (solvent control, 0.1% v/v) or different concentrations of the calpain inhibitor ALLN (B); after 2 days of treatment with Na azide (solvent control, 0.09% v/v) or monoclonal antibodies to activate (P5D2, 2.5 µg/mL) or inhibit (HMß1.1, 1.25 µg/mL) integrin-ß1 outside-in signaling (C); after 2 days of treatment with DMSO, FAK inhibitor (PF-00562271, 1 µM), or ROCK inhibitor (Y-27632, 1 µM, (D)). n/N = number of cells/ number of experiments.