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. 2021 Mar 16;26(6):1651. doi: 10.3390/molecules26061651

Figure 4.

Figure 4

Effects of HY7714 EPS on viability and ROS production of HS68 cells. (A) Effect of different concentrations (0.01, 0.1, 1, 5, and 10 μg/mL) of HY7714 EPS on viability of HS68 cells, as determined by MTT assay. (B) Cell viability of ultraviolet B (UVB)-induced HS68 cells at different concentrations (0.01, 0.1, 1, 5, and 10 μg/mL) of HY7714 EPS. Cells were irradiated with 30 mJ/m2 UVB. (C) Effect of HY7714 EPS on the level of ROS, as determined by DCFH-DA fluorescence. Representative image shows fluorescence staining of HS68 cells exposed to the indicated treatments. (D) Production of intracellular ROS was quantified using ImageJ. Data are expressed as means ± SD (n = 3), and values labeled with different letters are significantly different, p < 0.05 (a > b > c > d).