Table 2.
Sugar composition relative to Rha signal based on 1H-NMR data of bell pepper-PS and carrot-PS after extraction in hot water or bicarbonate (pH 7.5–8), and potency to enhance in vitro phagocytosis.
| Composition | Phagocytosis | |||||||
|---|---|---|---|---|---|---|---|---|
| Material | Extraction | GalA/Rha | Ara/Rha | Gal/Rha | GalA-meth | GalA-acet | 3 μg/mL | 30 μg/mL |
| bell pepper-PS | water | 6.9 | 4.4 | 0.0 | 50% | 20% | ++ | ++ |
| NaHCO3 | 5.3 | 0.0 | 0.0 | 0% | 0% | ++ | ++ | |
| carrot-PS | water | 10.9 | 11.8 | 5.3 | 60% | 20% | ++ | ++ |
| NaHCO3 | 7.6 | 0.0 | 5.3 | 0% | 0% | ++ | ++ | |
Monosaccharide ratios and degree of methylation are determined from the 1H-NMR spectra. In vitro phagocytosis stimulating activity is expressed as average normalized % phagocytosis of at least 2 blood donors, -: 0–20%, +/-: 20–40%, +: 40–80%, ++: >80% stimulation of positive control (LPS).