Figure 1.

(a–f) Analysis of L929 cell proliferation in the presence and absence of methionine. A total of 10,000 cells were seeded per well, incubated overnight and stimulated for 24 h, 48 h, 72 h and 120 h with or without methionine. The proliferation of the cells was analysed by crystal violet staining as described in the Materials and Methods. (a–d) The figures show a summary of the results obtained from four independent experiments (five values for every group). The control was normalized to 100%. (e,f) Simple analysis of potential cell death. Subfigures (e) and (f) show the results from one representative experiment. Unpaired Student’s t-test was used for comparison between control and Met (w/o) groups. p-values <0.05 indicated statistically significant effects. (ns: non-significant, *** p ≤ 0.001).