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(a–d) Methionine restriction sensitizes L929 cells to TNFα ligands and cisplatin. A total of 10,000 cells/well were seeded, incubated overnight and then stimulated for 72 h with or without methionine in combination with TNFα (a), FasL (b), TWEAK (c) or cisplatin (d). Cell proliferation was analysed by crystal violet staining as described in the Materials and Methods. The figures show a summary of the results from three independent experiments. The control was normalized to 100%.
