Table 2.
Formulations with composition of individual independent variables and observe responses of PLM-loaded glycerosome in Box–Behnken design.
| Formulation No | Independent Variables | Observe Responses | ||||
|---|---|---|---|---|---|---|
| X1 (mg) | X2 (% w/v) | X3 (% w/v) | Y1 (nm) | Y2 (%) | Y3 | |
| FL1 | 22.50 | 2.50 | 20.00 | 160.00 ± 16.30 | 74.00 ± 3.20 | 69.00 ± 6.80 |
| FL2 | 22.50 | 2.50 | 20.00 | 167.00 ± 17.10 | 73.00 ± 5.30 | 73.00 ± 6.10 |
| FL3 | 15.00 | 2.50 | 10.00 | 96.00 ± 9.40 | 63.00 ± 7.50 | 44.00 ± 4.30 |
| FL4 | 22.50 | 2.50 | 20.00 | 163.00 ± 15.20 | 77.00 ± 8.60 | 72.00 ± 6.50 |
| FL5 | 30.00 | 2.50 | 30.00 | 190.00 ± 18.30 | 84.00 ± 9.10 | 84.00 ± 9.90 |
| FL6 | 30.00 | 4.00 | 20.00 | 200.00 ± 18.40 | 82.00 ± 8.60 | 80.00 ± 8.60 |
| FL7 | 15.00 | 2.50 | 30.00 | 88.00 ± 6.60 | 68.00 ± 7.40 | 74.00 ± 5.80 |
| FL8 | 30.00 | 2.50 | 10.00 | 182.00 ± 14.40 | 65.00 ± 6.60 | 50.00 ± 4.60 |
| FL9 | 22.50 | 1.00 | 10.00 | 150.00 ± 12.70 | 61.00 ± 6.90 | 47.00 ± 4.60 |
| FL10 | 22.50 | 1.00 | 30.00 | 152.00 ± 10.50 | 71.00 ± 7.40 | 77.00 ± 8.60 |
| FL11 | 22.50 | 4.00 | 10.00 | 172.00 ± 16.80 | 59.00 ± 4.20 | 54.00 ± 5.90 |
| FL12 | 30.00 | 1.00 | 20.00 | 180.00 ± 15.00 | 78.00 ± 9.50 | 76.00 ± 9.50 |
| FL13 | 22.50 | 4.00 | 30.00 | 176.00 ± 16.80 | 80.00 ± 7.80 | 85.00 ± 9.90 |
| FL14 | 15.00 | 1.00 | 20.00 | 82.23 ± 4.70 | 67.00 ± 7.60 | 67.00 ± 6.70 |
| FL15 | 15.00 | 4.00 | 20.00 | 115.00 ± 8.50 | 73.00 ± 6.80 | 68.00 ± 7.90 |
| FL16 | 22.50 | 2.50 | 20.00 | 157.00 ± 10.80 | 78.00 ± 7.30 | 78.00 ± 8.80 |
| FL17 | 22.50 | 2.50 | 20.00 | 161.00 ± 13.50 | 75.30 ± 9.20 | 72.00 ± 8.30 |
X1: Phospholipid (mg); X2: Cholesterol (%w/v); X3: Glycerol (% w/v); Y1: Vesicles size (nm); Y2: Entrapment efficiency (%); Y3: Drug diffusion Flux (μg/cm2/h).