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. 2021 Mar 17;22(6):3056. doi: 10.3390/ijms22063056

Figure 3.

Figure 3

The expression and biological effects of GSTM1-5 by transient transfection expression. (A) The protein expression and cell viability after transfection. A total of 5637 cells were transfected with empty vectors or vectors with GSTM1-5 genes. After transfection for 24 h and 48 h, the proteins were detected by anti-DDK antibodies. Cell viability was analyzed after transfection for 32 h. (B) The protein expression and antibody characteristics. A total of 5637 cells were transfected with empty vectors or vectors with GSTM1-5 genes for 24 h; GSTM1~5 proteins were detected by their individual antibodies from GeneTex. (C) GST activity assay. A total of 5637 cells were transfected with empty vectors or vectors with GSTM1-5 genes for 24 h, the protein expression and GST activity were analyzed individually. (D) Cellular GSH level. A total of 5637 cells were transfected with empty vectors or vectors with GSTM1-5 genes for 24 h, the protein expression and GSH level (assayed by Cayman Chemical kit 703002) were analyzed. (E) Cellular GSH level in RT4 and BFTC 905 cells. After transient transfection for 24 h, cells were collected for GSH level detection (assayed by Cayman Chemical kit 703,002). All data are presented as the mean ± SE of three independent experiments. * p < 0.05; ** p < 0.01; *** p < 0.001.