. 2021 Mar 17;10(3):472. doi: 10.3390/antiox10030472

Table 3.

Profile of the specific polyphenols obtained by HPLE.

Polyphenols.	Temperature	Solvent
		Ethanol (15%)		Glycerol (15%)		Pure Water
		Mean	CV	Mean	CV	Mean	CV
Monomers (µg/g dw)
Gallic acid	90 °C	26.40 ^aA	0.08	29.15 ^aA	0.12	1.91 ^bA	0.03
	120 °C	58.32 ^aB	0.09	57.66 ^aB	0.11	2.20 ^bA	0.11
	150 °C	91.47 ^aC	0.10	99.26 ^aC	0.08	16.32 ^bB	0.12
Catechin	90 °C	33.17 ^bA	0.04	52.77 ^aA	0.04	26.16 ^bA	0.03
	120 °C	108.80 ^aB	0.10	117.05 ^aB	0.04	36.95 ^bB	0.14
	150 °C	179.55 ^aC	0.05	203.60 ^aC	0.06	126.93 ^bC	0.12
Epicatechin gallate	90 °C	9.98 ^aA	0.09	12.32 ^aA	0.13	3.20 ^bA	0.12
	120 °C	14.84 ^bB	0.04	20.52 ^aB	0.11	3.17 ^cA	0.07
	150 °C	31.66 ^bC	0.04	54.61 ^aC	0.06	17.49 ^cB	0.10
Quercetin	90 °C	30.08 ^bA	0.07	39.84 ^aA	0.12	6.07 ^cA	0.07
	120 °C	32.50 ^bA	0.03	55.46 ^aB	0.04	12.09 ^cB	0.07
	150 °C	41.45 ^bB	0.05	92.11 ^aC	0.07	26.92 ^cC	0.11
Epicatechin	90 °C	25.26 ^bA	0.06	65.76 ^aA	0.04	27.48 ^bA	0.08
	120 °C	140.77^{a B}	0.09	153.05 ^aB	0.05	33.59 ^bA	0.12
	150 °C	189.44 ^aC	0.14	162.45 ^aB	0.03	81.69 ^bB	0.15
Kaempferol	90 °C	1.68 ^bA	0.06	6.48 ^aA	0.12	0.47 ^cA	0.03
	120 °C	5.63 ^bB	0.10	8.72 ^aA	0.07	0.66 ^cB	0.03
	150 °C	15.29 ^bC	0.08	20.02 ^aB	0.04	1.18 ^cC	0.04
Rutin	90 °C	1.10 ^aA	0.06	1.75 ^aA	0.04	0.42 ^cA	0.04
	120 °C	1.99 ^aB	0.07	2.37 ^aB	0.10	0.65 ^bB	0.05
	150 °C	ND		ND		ND
Dimers (µg/g dw)
Procyanidin B1	90 °C	36.55 ^bB	0.03	43.08 ^aB	0.08	14.92 ^cB	0.11
	120 °C	52.11 ^aC	0.14	65.99 ^aC	0.13	12.75 ^bB	0.08
	150 °C	10.03 ^bA	0.07	35.08 ^aA	0.11	4.05 ^cA	0.11
Procyanidin B2	90 °C	57.63 ^bB	0.03	72.05 ^aB	0.07	37.29 ^cB	0.03
	120 °C	58.06 ^bB	0.04	87.94 ^aC	0.04	14.26 ^cA	0.04
	150 °C	42.56 ^bA	0.11	61.08 ^aA	0.10	13.04 ^cA	0.06
Trimers (µg/g dw)
Procyanidin C1	90 °C	17.32 ^aB	0.07	13.46 ^aB	0.09	2.13 ^bB	0.03
	120 °C	12.56 ^aA	0.07	6.49 ^bA	0.11	1.47 ^cA	0.09
	150 °C	6.95 ^aC	0.11	5.95 ^aA	0.06	ND
Procyanidin B2 gallate	90 °C	55.81 ^aA	0.08	48.78 ^aB	0.06	14.95 ^bC	0.12
	120 °C	49.17 ^aA	0.14	42.21 ^aB	0.12	6.47 ^bB	0.08
	150 °C	25.32 ^aB	0.06	16.24 ^bA	0.08	4.13^cA	0.08
Tetramers (µg/g dw)
Procyanidin B2 di-O-gallate	90 °C	17.13 ^aC	0.07	14.97 ^bC	0.06	4.35 ^cB	0.07
	120 °C	12.65 ^aB	0.09	7.56 ^bB	0.07	1.89 ^cA	0.11
	150 °C	7.21 ^aA	0.04	3.64 ^bA	0.07	ND

The results are expressed as µg of specific polyphenols per gram dry weight. CV: variation coefficient. The different capital letters in the same column show the significant differences (p < 0.05) between the different HPLE temperatures for the same HPLE solvent, while the different low-case letters in the same row show the significant differences (p < 0.05) between the different extraction solvents at the same HPLE temperature.