Skip to main content
. 2021 Mar 17;9(3):309. doi: 10.3390/biomedicines9030309

Figure 4.

Figure 4

Effects of prokineticin antagonists’ on placental structure and trophoblasts invasion. (A) depicts analysis of the placental zones of CTL, PC7 and PKRA placentas. For each group, at least three placental sections/animal were analyzed. The graph shows proportions of the surface layer of 3 zones of the placenta (labyrinth, junctional zone, and decidua). Surfaces of the 3 layers were measured on parasagittal sections for each placenta. Mean values were used to calculate the mean (SEM) surface proportion of the layers. p < 0.05. NS, not significant. (B) shows representative photomicrographs of placental sections stained with Peroxide Acid Shiff at different magnifications. Placentas were collected from CTL, PC7 and PKRA treated mice at 12.5 dpc. JZ: Junctional Zone; ITC: Invasive Trophoblast Cell; mBv: Maternal Blood Vessel. (C) depicts a graph that compares the number glycogenic cells in the maternal decidua. (D,E) shows western blot analysis and quantification of CA9 protein levels in the placenta collected from CTL, PC7 and PKRA treated mice. Standardization of immunoreactivity was performed using antibodies against β-actin. Data are represented as mean ± SEM. p < 0.05. Bars with different letters are significantly different from each other.