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. 2021 Mar 17;9(3):308. doi: 10.3390/biomedicines9030308

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Migration of the granulation tissue. (A,B) representative histological slides showing the migration of the granulation tissue within cylinders filled with DBBM-C soaked in NaCl 0.9% at 2 weeks and 4 weeks (dotted black line, mean height of the granulation tissue (GT); dotted green line, inferior limit of the osteogenic zone (OZ) upper limit of the remodeling zone (RZ). Yellow box and arrow: magnification on the picture indicated by the arrow. (C) Higher magnification of the granulation tissue showing mononuclear cells surrounding the particles of DBBM-C. (D) Box plot showing the mean distance (mm) between the bone bed and the GT in the cylinders filled with DBBM-C scaffolds either pre-treated with blood (dark grey) or NaCl 0.9% (light grey) at 2, 4, 8 and 12 wks. Horizontal line: median; Boxes: 25–75%; ×: mean; Bars: range of non-outliers.