Table 1.

The RT-qPCR results from ethylenediamine tetra-acetic acid (EDTA) blood from Cat 1 and mucosal, fecal, fur, and environmental swabs from both cats.

Cat ID	Collection Date	Material	E-Assay (CT-Value) 1	RdRp-Assay (CT-Value) 1	Interpretation of the Results 2
Cat 1	10 November 2020	EDTA blood	43.2	42.2	questionable positive
	16 November 2020	oral swab	negative	36.6	negative
		nasal swab	32.6	33.2	Positive
		fecal swab	negative	44	negative
		fur swab	34.4	34.1	positive
		bedding swab	31.8	31.7	positive
	19 November 2020	oral swab	negative	negative	negative
		nasal swab	32.5	32.4	positive
		fecal swab	negative	negative	negative
		fur swab	36.9	35.7	positive
Cat 2	16 November 2020	oral swab	37.5	36.2	positive
		nasal swab	negative	negative	negative
		fecal swab	negative	negative	negative
		fur swab	44.9	35.8	questionable positive
		bedding swab	32.9	33.1	positive
	19 November 2020	oral swab	negative	negative	negative
		nasal swab	negative	negative	negative
		fecal swab	negative	negative	negative
		fur swab	33.8	35.9	positive

¹ Displayed cycle threshold (CT) values indicate measurements in neat samples except for the oral swab from Cat 2 (dilution 1:5) collected on 16 November 2020. ² Interpretation: positive: CT-values ≤ 38 in both assays; questionable positive: CT-values between >38 and <45 in one or both assays; and negative: CT-values ≥ 45 in one or both assays.