Figure 1.

Chrysophanol (CHR) does not show cytotoxicity on HT-29 colorectal cells. (A) The chemical structure of chrysophanol. (B) HT-29 cells (1 × 10⁴/well, 96-well plate) were treated with the indicated concentrations of chrysophanol (0 to 40 μM) for 24 h and methyl thiazol tetrazolium (MTT) assay was performed for determination of cell viability. (C,D) HT-29 cells (1 × 10⁴/well, 96-well plate) were treated with the indicated concentration (0 to 40 μM) of chrysophanol for 24 h and the intensity of AnnexinV and Caspase3/7 was assessed by IncuCyte imaging system. Obtained cell images (C) and intensity of AnnexinV or Caspase3/7 is presented in bar graph (% of control). DIC; differential interference contrast. (D) The mean value of three experiments ±SEM is presented. SEM; standard error of the mean.