Figure 6.

Polarized microscope images of 3D bioengineered scaffold stained by Sirius red. The collagen synthetic matrix is represented by network fibers stained in light red. This collagen matrix underwent a progressive degradation that was larger and more abundant in dynamic culture conditions. Furthermore, we observed new matrix deposition (darker red areas) within the hollow areas of the scaffold, but mainly at day 16 in the scaffold cultured in perfusion.). All images are obtained using an enlargements of 40×.