Table 2.
List of additional primary and secondary antibodies used for confocal microscopy.
| Marker | Host | Reactivity | Clone | Conjugate | Company |
|---|---|---|---|---|---|
| CD49f | rat IgG1 | rabbit | GoH3 | - | Biolegend |
| Sec. Ab | goat | rat | polyclonal | FITC | Biolegend |
| ALPL | mouse | rabbit | TRA-2-49 | - | Novus Biologicals |
| SOX2 | mouse | human | 245610 | - | R&D Systems |
| Sec. Ab | goat | mouse IgG | polyclonal | FITC | Bio-Rad |
| NANOG | goat | human | polyclonal | - | R&D Systems |
| OCT4 | goat | human | polyclonal | - | R&D Systems |
| Sec. Ab | donkey | goat IgG | polyclonal | FITC | Bio-Rad |
Sec. Ab—secondary antibody; ALPL—alkaline phosphatase; SOX2—sex determining region Y—box 2; OCT4—octamer-binding transcription factor 4.