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. 2021 Mar 17;9(3):622. doi: 10.3390/microorganisms9030622

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Cytokines and chemokines mRNA expression by Aggregatibacter actinomycetemcomitans-induced oral epithelial cells (OKF6/TERT2). Expression in oral epithelial cells infected at a MOI = 10² with strains ATCC^® 43717^TM (serotype a), ATCC^® 43718^TM (serotype b), and ATCC^® 43719^TM (serotype c), 2 and 24 h after infection. For relative expression, mRNA expression in non-infected (ni) oral epithelial cells was considered as 1, as a reference for fold-change in expression using 18S rRNA expression levels as a normalizing endogenous control. Data are represented as fold-change means and standard deviation of three independent experiments performed in duplicate. (A) IL-1β, (B) IL-6, (C) IL-8, (D) IL-18, and (E) TNF-α. Asterisks were used to indicate a value of p considered statistically significant (* p < 0.05).