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. 2021 Mar 17;11(3):537. doi: 10.3390/diagnostics11030537

Figure 5.

Figure 5

Purification and confirmation of recombinant S protein of SARS-CoV-2 expressed in Drosophila Schneider’s line 2 (S2) cells. (A) Purification of recombinant S under different conditions. M: marker, 1: 30 mM imidazole, 2: 60 mM imidazole, 3: 80 mM imidazole, 4: 250 mM imidazole. (B) Western blot analysis of purified S protein. M: marker; 1: negative control; 2: transient expression of recombinant S in Drosophila expression system; 3–6: expression of recombinant S1 in stable cell lines, 1 µL, 2 µL, 5 µL, and 10 µL.