Table 2.
Fatty acid profile of the dark chocolate.
| Fatty Acids | Chocolate Samples | |||
|---|---|---|---|---|
| S1 | S2 | S3 | S4 | |
| Fatty Acids Concentration, % from Total Fat Content | ||||
| C4:0 | nd | nd | nd | nd |
| C6:0 | nd | nd | nd | nd |
| C8:0 | nd | nd | nd | 0.336 ± 0.028 |
| C10:0 | nd | nd | nd | 0.278 ± 0.054 |
| C11:0 | nd | nd | nd | nd |
| C12:0 | nd | nd | nd | 3.309 ± 0.986 |
| C13:0 | nd | nd | nd | nd |
| C14:0 | 0.051 ± 0.007 a | 0.068 ± 0.004 c | 0.057 ± 0.003 b | 1.419 ± 0.096 d |
| C14:1 | nd | nd | nd | nd |
| C15:0 | nd | nd | nd | nd |
| C15:1 | nd | nd | nd | nd |
| C16:0 | 25.868 ± 0.101 b | 23.211 ± 0.028 a | 25.948 ± 0.114 b | 25.738 ± 0.191 b |
| C16:1 | 0.100 ± 0.009 a | 0.132 ± 0.012 b | 0.143 ± 0.011 b | 0.148 ± 0.018 b |
| C17:0 | 0.144 ± 0.012 a | 0.130 ± 0.018 a | 0.176 ± 0.010 b | 0.207 ± 0.014 c |
| C17:1 | nd | nd | nd | nd |
| C18:0 | 32.553 ± 0.156 b | 33.659 ± 0.418 c | 33.022 ± 0.318 c | 29.581 ± 0.128 a |
| C18:0 cis. trans | 32.850 ± 0.110 c | 32.973 ± 0.132 c | 32.401 ± 0.118 b | 30.965 ± 0.098 a |
| C18:2 | 7.153 ± 0.078 d | 6.917 ± 0.097 c | 4.121 ± 0.234 a | 6.429 ± 0.118 b |
| C18:2 trans | nd | nd | nd | nd |
| C18:3ɣ | nd | 0.06 ± 0.01 | nd | nd |
| C18:3α | 0.379 ± 0.118 a | 1.730 ± 0.118 c | 3.256 ± 0.769 d | 0.647 ± 0.237 b |
| C20:0 | 0.618 ± 0.008 a | 0.832 ± 0.019 c | 0.654 ± 0.004 c | 0.639 ± 0.009 b |
| C20:1 | 0.053 ± 0.011 a | 0.125 ± 0.014 c | 0.055 ± 0.031 a | 0.058 ± 0.024 a |
| C20:2 | nd | nd | nd | nd |
| C20:3 | nd | nd | nd | nd |
| C21:0 | nd | nd | nd | nd |
| C20:4 | nd | nd | nd | nd |
| C20:3 | nd | nd | nd | nd |
| C20:5 | nd | nd | nd | nd |
| C22:0 | 0.158 ± 0.003 d | 0.110 ± 0.005 b | 0.094 ± 0.008 a | 0.148 ± 0.005 c |
| C22:1 | nd | nd | nd | nd |
| C22:2 | nd | nd | nd | nd |
| C23:0 | nd | nd | nd | nd |
| C24:0 | 0.073 ± 0.006 b | 0.053 ± 0.011 a | 0.073 ± 0.008 b | 0.098 ± 0.010 c |
| C22:6 | nd | nd | nd | nd |
| C24:1 | nd | nd | nd | nd |
| SFAs | 59.465 | 58.063 | 60.024 | 61.753 |
| MUFAs | 33.003 | 33.23 | 32.599 | 31.171 |
| PUFAs | 7.532 | 8.707 | 7.377 | 7.076 |
| Omega3 | 0.379 | 1.73 | 3.256 | 0.647 |
| Omega6 | 7.153 | 6.977 | 4.121 | 6.429 |
| Omega 9 | 33.003 | 33.23 | 32.599 | 31.171 |
C4:0—methyl butyrate; C6:0—methyl hexonoate; C8:0—methyl octanoate; C10:0—methyl decanoate; C11:0—methyl undecanoate; C12:0—methyl laurate; C13:0—methyl tridecanoate; C14:0—methyl tetradecanoate; C14:1—methyl myristoleate; C15:0—methyl pentadecanoate; C15:1—cis-10-pentadecenoic acid methyl ester; C16:0—methyl palmitate; C16:1—methyl palmitoleate; C17:0—methyl heptadecanoate; C17:1—cis-10-heptadecanoic acid methyl ester; C18:0—methyl stearate; C18:0 cis. trans—cis.trans-9- oleic acid methyl ester; C18:2—methyl linoleate; C18:2 trans–linolelaidic acid methyl ester; C18:3ɣ—gamma- linolenic acid methyl ester; C18:3α—alfa linolenic acid methyl ester; C20:0—eicosanoic acid methyl ester; C20:1—cis-11-eicosenoic acid methyl ester; C20:2—cis-11.14-eicosadienoic acid methyl ester; C20:3—cis-8.11.14-eicosatrienoic acid methyl ester; C21:0—methyl heinecosonoate; C20:4—cis-5.8.11.14-eicosatetraenoic acid methyl ester; C20:3—cis-11.14.17-eicosatrienoic acid methyl ester; C20:5—cis-5.8.11.14.17-eicosapentanoic acid methyl ester; C22:0—methyl docosanoate; C22:1—cis-13-docosenoic acid methyl ester; C22:2—cis-13.16-docosadienoic acid methyl ester; C23:0—methyl tricosanoate; C24:0—methyl tetracosanoate; C22:6—all cis-4.7.10.13.16.19-docosahexanoic acid methyl ester; C24:1—cis-15-tetracosenoic acid methyl ester; SFAs—saturated fatty acids ; MUFAs—monounsaturated fatty acids; PUFAs—polyunsaturated fatty acids. Data expressed as a mean value (n = 3) ± standard deviation. a–d—Means with different letters within a row are significantly different (p ≤ 0.05).