Table 1.
Plasmids and bacterial strains used in this study.
| Plasmid | Description | Antibiotic Resistance (µg/mL) | Reference/Source |
|---|---|---|---|
| pK18mobsecB | Suicide vector for homologous recombination | Km50 | [23] |
| pK18dohrR | pK18mobsecB contains the upstream and downstream regions of ohrR-C | Km50 | This study |
| pK18dohr | pK18mobsecB contains the upstream and downstream regions of ohr-C | Km50 | This study |
| pK18dohrRohr | pK18mobsecB contains the upstream and downstream regions of ohrR-C-ohr-C | Km50 | This study |
| pQE80L | Expression vector with colE1 origin for His-tag fusion protein purification | Amp50 | Qiagen |
| pOhrR-C | ohrR-C gene with N-terminal-fused His-tag in pQE80L | Amp50 | This study |
| pOhrR-C-Pohr-ohr-C | ohr-C and ohrR-C gene promoter was cloned downstream of pOhrR-C | Amp50 | This study |
| Strain | Description | Reference/Source | |
| E. coli DH5α | F−, supE44, hsdR17, recA1, gyrA96, endA1, thi-1, relA1, deoR, λ− | ATCC 53868 | |
| E. coli S17-1λpir | thi-, thr , leu , tonA , lacY , supE , recA::RP4-2-Tc::Mu, Smr, lpir | [23] | |
| Acinetobacter baumannii ATCC 19606 | Primary strain used in this study | [19] | |
|
Acinetobacter baumannii ATCC 17978 |
Most studied strain to date | [14] | |
| ∆ohrR | Marker-less ohrR-c deletion mutant | This study | |
| ∆ohr | Marker-less ohr-c deletion mutant | This study | |
| ∆ohrRohr | Marker-less ohrR-c-ohr-c deletion mutant | This study | |
Amp: ampicillin; Km: kanamycin.