Table 1.
Primer pairs used in this study to complete the near-whole-genome sequencing of human metapneumovirus.
| Assay | Primer Name | Sequence (5’-3’) | Position (nt) * | PCR Amplicon Size (bp) |
|---|---|---|---|---|
| PCR1 | HMPV1_F | GGGACAAATAAAAATGTCTCTTCA | 41 | 4125 |
| HMPV1_R | CTTCCTGTGCTRACYTTRCA | 4165 | ||
| PCR2 | HMPV2_F | ACAGCAGCRGGRATYAATGT | 4074 | 4010 |
| HMPV2_R | TAGTACTGAAYTGAGCATGYTCAG | 8083 | ||
| PCR3 | HMPV3_F | AACTGTTAACATGGAAAGATGTGATG | 7823 | 3229 |
| HMPV3_R | TAAGCTGGAACWGAWGCTG | 11,051 | ||
| PCR4 | HMPV4_F | TCAATAGGGAGTCTRTGTCARGAA | 9730 | 3675 |
| HMPV4_R | GRCAAAAAAACCGTATACATYC | 13,404 |
* Nucleotide positions are relative to strain A1 (NCBI GenBank Accession KU821121).