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. 2021 Mar 17;22(6):3091. doi: 10.3390/ijms22063091

Figure 4.

Figure 4

O-1602 induces activation of Akt through GPR55 and PI3K. Hep3B cells were treated with different concentrations of O-1602 for 48 h. Western blotting analysis was performed for Akt and phospho-Akt (S347) in Hep3B cells. (A) Representative blots of phospho-Akt, Akt, and β-actin. (B) Quantitative western blot analysis of phospho-Akt. Hep3B cells were pretreated with CID16020046 or LY294002 for 30 min and then treated with 10 μM O-1602 for 48 h. (C) Representative blots of phospho-Akt, Akt, and β-actin. (D) Quantitative western blot analysis of phospho-Akt. Data are from three individual experiments and expressed as mean ± SD. ** p < 0.01, *** p < 0.001, compared with the non-treated group; # p < 0.05, compared with O-1602-treated group.