Table 2.
The screened marker compounds for the discrimination of the different parts of P. ginseng root.
| Source | Peak No | tR (min) | Observed m/z | Adducts | Aglycone Type | Identification | Main Existing Groups |
|---|---|---|---|---|---|---|---|
| MR and FR | 20 | 6.31 | 991.5446 | +HCOO | PPT | Ginsenoside Re | Fibrous root |
| 24 | 7.23 | 1033.5545 | +HCOO | PPT | 6′′′-O-Acetyl-ginsenoside Re/isomer | Fibrous root | |
| 39 | 9.15 | 845.4881 | +HCOO | PPT | Ginsenoside Rf | Fibrous root | |
| 46 | 10.58 | 829.4936 | +HCOO | PPT | Ginsenoside Rg2 | Fibrous root | |
| 48 | 11.19 | 1255.6295 | +HCOO | PPD | Ginsenoside Ra2 | Fibrous root | |
| 50 | 11.49 | 1153.5979 | +HCOO | PPD | Ginsenoside Rb1 | Fibrous root | |
| 51 | 11.66 | 1297.6387 | +HCOO | PPD | Ginsenoside Ra5/(3β, 12β)-3-[[2-O-(6-O-Acetyl-β-D-glucopyranosyl)-β-D-glucopyranosyl]oxy]-12-hydroxydammar-24-en-20-yl O-β-D-xylopyranosyl-(1→2)-O-α-L-arabinopyranosyl-(1→6)-β-D-glucopyranoside or isomer | Fibrous root | |
| 54 | 11.99 | 1195.6074 | +HCOO | PPD | Quinquenoside R1/(3β, 12β)-20-[[6-O-(6-O-Acetyl-β-D-glucopyranosyl)-β-D-glucopyranosyl]oxy]-12-hydroxydammar-24-en-3-yl 2-O-β-D-glucopyranosyl-β-D-glucopyranoside or isomer | Fibrous root | |
| 56 | 12.42 | 1123.5869 | +HCOO | PPD | Ginsenoside Rc | Fibrous root | |
| 59 | 13.00 | 1165.5969 | +HCOO | PPD | Ginsenoside Rs1/Ginsenoside Rs2/Pseudoginsenoside F8 isomer | Fibrous root | |
| 61 | 13.32 | 1195.6077 | +HCOO | PPD | Quinquenoside R1/(3β, 12β)-20-[[6-O-(6-O-Acetyl-β-D-glucopyranosyl)-β-D-glucopyranosyl]oxy]-12-hydroxydammar-24-en-3-yl 2-O-β-D-glucopyranosyl-β-D-glucopyranoside or isomer | Fibrous root | |
| 63 | 13.43 | 1123.5865 | +HCOO | PPD | Ginsenoside Rb2 | Fibrous root | |
| 65 | 13.78 | 1123.5871 | +HCOO | PPD | Ginsenoside Rb3 | Fibrous root | |
| 67 | 14.00 | 1165.597 | +HCOO | PPD | Ginsenoside Rs1/Ginsenoside Rs2/Pseudoginsenoside F8 isomer | Fibrous root | |
| 69 | 14.36 | 1165.5972 | +HCOO | PPD | Ginsenoside Rs1/Ginsenoside Rs2/Pseudoginsenoside F8 isomer | Fibrous root | |
| 77 | 15.60 | 1165.5974 | +HCOO | PPD | Ginsenoside Rs1/Ginsenoside Rs2/Pseudoginsenoside F8 isomer | Fibrous root | |
| 79 | 15.82 | 991.5443 | +HCOO | PPD | Ginsenoside Rd | Fibrous root | |
| 80 | 16.50 | 1033.5548 | +HCOO | PPD | Pseudoginsenoside Rc1/Quinquenoside III/β-D-Glucopyranoside, (3β, 12β)-3-(β-D-glucopyranosyloxy)-12-hydroxydammar-24-en-20-yl 6-O-(6-O-acetyl-β-D-glucopyranosyl)-(9CI) or isomer | Fibrous root | |
| 81 | 16.92 | 1033.5549 | +HCOO | PPD | Pseudoginsenoside Rc1/Quinquenoside III/β-D-Glucopyranoside, (3β, 12β)-3-(β-D-glucopyranosyloxy)-12-hydroxydammar-24-en-20-yl 6-O-(6-O-acetyl-β-D-glucopyranosyl)-(9CI) or isomer | Fibrous root | |
| 86 | 17.84 | 1033.555 | +HCOO | PPD | Pseudoginsenoside Rc1/Quinquenoside III/β-D-Glucopyranoside, (3β, 12β)-3-(β-D-glucopyranosyloxy)-12-hydroxydammar-24-en-20-yl 6-O-(6-O-acetyl-β-D-glucopyranosyl)-(9CI) or isomer | Fibrous root | |
| MR and BR | 20 | 6.31 | 991.5446 | +HCOO | PPT | Ginsenoside Re | Branch root |
| 24 | 7.23 | 1033.5545 | +HCOO | PPT | 6′′′-O-Acetyl-ginsenoside Re/isomer | Branch root | |
| 39 | 9.15 | 845.4881 | +HCOO | PPT | Ginsenoside Rf | Branch root | |
| 46 | 10.58 | 829.4936 | +HCOO | PPT | Ginsenoside Rg2 | Branch root | |
| 50 | 11.49 | 1153.5979 | +HCOO | PPD | Ginsenoside Rb1 | Branch root | |
| 54 | 11.99 | 1195.6074 | +HCOO | PPD | Quinquenoside R1/(3β, 12β)-20-[[6-O-(6-O-Acetyl-β-D-glucopyranosyl)-β-D-glucopyranosyl]oxy]-12-hydroxydammar-24-en-3-yl 2-O-β-D-glucopyranosyl-β-D-glucopyranoside or isomer | Branch root | |
| 56 | 12.42 | 1123.5869 | +HCOO | PPD | Ginsenoside Rc | Branch root | |
| 59 | 13.00 | 1165.5969 | +HCOO | PPD | Ginsenoside Rs1/Ginsenoside Rs2/Pseudoginsenoside F8 isomer | Branch root | |
| 61 | 13.32 | 1195.6077 | +HCOO | PPD | Quinquenoside R1/(3β, 12β)-20-[[6-O-(6-O-Acetyl-β-D-glucopyranosyl)-β-D-glucopyranosyl]oxy]-12-hydroxydammar-24-en-3-yl 2-O-β-D-glucopyranosyl-β-D-glucopyranoside or isomer | Branch root | |
| 63 | 13.43 | 1123.5865 | +HCOO | PPD | Ginsenoside Rb2 | Branch root | |
| 67 | 14.00 | 1165.597 | +HCOO | PPD | Ginsenoside Rs1/Ginsenoside Rs2/Pseudoginsenoside F8 isomer | Branch root | |
| 69 | 14.36 | 1165.5972 | +HCOO | PPD | Ginsenoside Rs1/Ginsenoside Rs2/Pseudoginsenoside F8 isomer | Branch root | |
| 77 | 15.60 | 1165.5974 | +HCOO | PPD | Ginsenoside Rs1/Ginsenoside Rs2/Pseudoginsenoside F8 isomer | Branch root | |
| 79 | 15.82 | 991.5443 | +HCOO | PPD | Ginsenoside Rd | Branch root | |
| 80 | 16.50 | 1033.5548 | +HCOO | PPD | Pseudoginsenoside Rc1/Quinquenoside III/β-D-Glucopyranoside, (3β, 12β)-3-(β-D-glucopyranosyloxy)-12-hydroxydammar-24-en-20-yl 6-O-(6-O-acetyl-β-D-glucopyranosyl)-(9CI) or isomer | Branch root | |
| 81 | 16.92 | 1033.5549 | +HCOO | PPD | Pseudoginsenoside Rc1/Quinquenoside III/β-D-Glucopyranoside, (3β, 12β)-3-(β-D-glucopyranosyloxy)-12-hydroxydammar-24-en-20-yl 6-O-(6-O-acetyl-β-D-glucopyranosyl)-(9CI) or isomer | Branch root | |
| 86 | 17.84 | 1033.555 | +HCOO | PPD | Pseudoginsenoside Rc1/Quinquenoside III/β-D-Glucopyranoside, (3β, 12β)-3-(β-D-glucopyranosyloxy)-12-hydroxydammar-24-en-20-yl 6-O-(6-O-acetyl-β-D-glucopyranosyl)-(9CI) or isomer | Branch root | |
| MR and RH | 20 | 6.31 | 991.5446 | +HCOO | PPT | Ginsenoside Re | Rhizome |
| 24 | 7.23 | 1033.5545 | +HCOO | PPT | 6′′′-O-Acetyl-ginsenoside Re/isomer | Rhizome | |
| 39 | 9.15 | 845.4881 | +HCOO | PPT | Ginsenoside Rf | Rhizome | |
| 46 | 10.58 | 829.4936 | +HCOO | PPT | Ginsenoside Rg2 | Rhizome | |
| 50 | 11.49 | 1153.5979 | +HCOO | PPD | Ginsenoside Rb1 | Rhizome | |
| 53 | 11.93 | 955.4868 | +HCOO | OA | Ginsenoside Ro | Rhizome | |
| 54 | 11.99 | 1195.6074 | +HCOO | PPD | Quinquenoside R1/(3β, 12β)-20-[[6-O-(6-O-Acetyl-β-D-glucopyranosyl)-β-D-glucopyranosyl]oxy]-12-hydroxydammar-24-en-3-yl 2-O-β-D-glucopyranosyl-β-D-glucopyranoside or isomer | Rhizome | |
| 56 | 12.42 | 1123.5869 | +HCOO | PPD | Ginsenoside Rc | Rhizome | |
| 59 | 13.00 | 1165.5969 | +HCOO | PPD | Ginsenoside Rs1/Ginsenoside Rs2/Pseudoginsenoside F8 isomer | Rhizome | |
| 61 | 13.32 | 1195.6077 | +HCOO | PPD | Quinquenoside R1/(3β, 12β)-20-[[6-O-(6-O-Acetyl-β-D-glucopyranosyl)-β-D-glucopyranosyl]oxy]-12-hydroxydammar-24-en-3-yl 2-O-β-D-glucopyranosyl-β-D-glucopyranoside or isomer | Rhizome | |
| 63 | 13.43 | 1123.5865 | +HCOO | PPD | Ginsenoside Rb2 | Rhizome | |
| 67 | 14.00 | 1165.597 | +HCOO | PPD | Ginsenoside Rs1/Ginsenoside Rs2/Pseudoginsenoside F8 isomer | Rhizome | |
| 69 | 14.36 | 1165.5972 | +HCOO | PPD | Ginsenoside Rs1/Ginsenoside Rs2/Pseudoginsenoside F8 isomer | Rhizome | |
| 75 | 14.93 | 793.4361 | −H | OA | Chikusetsusaponin Iva | Rhizome | |
| 77 | 15.60 | 1165.5974 | +HCOO | PPD | Ginsenoside Rs1/Ginsenoside Rs2/Pseudoginsenoside F8 isomer | Rhizome | |
| 79 | 15.82 | 991.5443 | +HCOO | PPD | Ginsenoside Rd | Rhizome | |
| 80 | 16.50 | 1033.5548 | +HCOO | PPD | Pseudoginsenoside Rc1/Quinquenoside III/β-D-Glucopyranoside, (3β, 12β)-3-(β-D-glucopyranosyloxy)-12-hydroxydammar-24-en-20-yl 6-O-(6-O-acetyl-β-D-glucopyranosyl)-(9CI) or isomer | Rhizome | |
| 81 | 16.92 | 1033.5549 | +HCOO | PPD | Pseudoginsenoside Rc1/Quinquenoside III/β-D-Glucopyranoside, (3β, 12β)-3-(β-D-glucopyranosyloxy)-12-hydroxydammar-24-en-20-yl 6-O-(6-O-acetyl-β-D-glucopyranosyl)-(9CI) or isomer | Rhizome | |
| 86 | 17.84 | 1033.5550 | +HCOO | PPD | Pseudoginsenoside Rc1/Quinquenoside III/β-D-Glucopyranoside, (3β, 12β)-3-(β-D-glucopyranosyloxy)-12-hydroxydammar-24-en-20-yl 6-O-(6-oot niteO-acetyl-β-D-glucopyranosyl)-(9CI) or isomer | Rhizome |
Peak numbers were consistent with Table 1.