Figure 4.

SAC enhances testosterone production in I-10 cells. (A) Cells at a density of 3.6 × 10⁴ cells/well were incubated in 96-well plates for 24 h with different concentrations of SAC (0, 1, 10, and 100 µM). Cell proliferation was determined by the water-soluble tetrazolium salts-1 (WST-1) assay. Data are presented as the mean ± SE (n = 4–5). Data were analyzed by one-way ANOVA. (B) Cells at a density of 6 × 10⁴ cells/well were incubated in 12-well plates with SAC for 24 h. Testosterone was measured from the medium by the EIA method. Data are presented as the mean ± SE (n = 3). Data were analyzed by Dunnett’s test. * p < 0.05 and ** p < 0.01 vs. 0 µM.