Figure 2.

Synergistic cell death induced by PAT and CdCl₂ is mitochondria-mediated caspase-dependent apoptosis. (A) Effect of PAT/CdCl₂ co-administration on mitochondrial permeability transition (MPT) in AML12 cells. The cells were treated with PAT, CdCl₂ or their combination for 12 h and the MPT was detected by tetraethyl benzimidazolyl carbocyanine iodide (JC-1) staining. (B) Effect of PAT/CdCl₂ co-administration on mitochondria-mediated apoptosis. AML12 cells were incubated with PAT with or without CdCl₂ for 24 h, and then, Bim and caspase-9 were assessed. (C) Effect of PAT/CdCl₂ co-administration on mitochondria-mediated apoptosis. HEK293 cells were incubated with PAT with or without CdCl₂ for 24 h, and then, Bim and caspase-9 were assessed. n = 3, * P < 0.05.