Figure 5.

JNK1 inhibitor (DB07268) can rescue the synergistic effect caused by co-treatment with PAT and CdCl₂. (A) Effect of DB07268 on PAT/CdCl₂-induced cell death. The cells were treated with the combination of PAT and CdCl₂ or the combination plus DB07268 for 24 h, and then, cell death was measured. (B) Effect of DB07268 on PAT/CdCl₂-induced ROS generation. The cells were incubated with the combination of PAT and CdCl₂ or the combination plus DB07268 for 12 h, and ROS levels were detected by using DCFH-DA. (C) Effect of DB07268 on activation of p53 and Bim induced by PAT and CdCl₂. AML12 cells were incubated with the combination of PAT and CdCl₂ or the combination plus DB07268 for 24 h, and then, p53, p-p53 and Bim were measured by Western blotting. n = 3, * P < 0.05 and *** P < 0.001.