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. 2021 Mar 19;10(3):586. doi: 10.3390/plants10030586

Figure 3.

Figure 3

Effect of BF-L on NF-κB signaling activation in LPS-stimulated RAW264.7 cells. (a) RAW264.7 cells were pretreated with BF-L for 6 h and then co-treated with LPS (1 μg/mL) for 30 min. For Western blot analysis, the cell lysates were subjected to SDS-PAGE and the Western blot was performed using antibodies against p-IκB-α and IκB-α. β-actin was used as internal control. (b) RAW264.7 cells were pretreated with BR-L for 6 h and then co-treated with LPS (1 μg/mL) for 30 min. After the treatment, the nucleus fraction was prepared. For Western blot analysis, the cell lysates were subjected to SDS-PAGE and the Western blot was performed using antibodies against p65. Actin was used as internal control for Western blot analysis.