Table 1.

Phenotypic characterization of Catonella massiliensis sp. nov., compared to other closely related species. +, Positive; w, weak; -, negative; ND, no data available. Strains: 1, Marseille-Q4567^T; 2, Cuneatibacter caecimuris DSM 29486^T [17]; 3, Faecalicatena orotica JCM 1429^T [18]; 4, Faecalicatena contorta JCM 6483^T [18]; 5, Faecalicatena fissicatena JCM 31501^T [18]; 6, Anaerocolumna cellulosilytica strain SN021^T [19]; 7, Catonella morbi ATCC 51271^T [13]; 8, Herbinix luporum strain SD1D^T [20].

Characteristic	1	2	3	4	5	6	7	8
Gram stain	-	+	+	+	+	+	-	+
Spore formation	-	-	+	-	-	+	-	-
Temperature (°C)	28–41.5	37	20–40	20–40	20–40	15–40	37	40–65
pH	6.5–8.5	7.0	6.0–8.0	6.0–8.0	6.0–8.0	6.2–8.5	5.0–5.6	6.5–8.5
Catalase activity	-	ND	ND	ND	-	-	-	-
Oxidase activity	-	ND	ND	ND	ND	-	ND	ND
Acid produced from:
L-Arabinose	+	+	+	+	+	+	-	+
Cellobiose	+	-	+	+	-	+	+	+
Lactose	+	-	+	+	-	+	+	-
Maltose	+	-	+	+	+	+	+	ND
D-Mannitol	+	-	+	-	-	-	-	ND
D-Mannose	+	-	+	+	+	+	-	+
Raffinose	+	-	+	+	-	-	+	ND
L-Rhamnose	+	+	+	+	+	-	+	ND
D-Sorbitol	+	-	+	-	-	-	-	-
Enzyme activity:
Alkaline phosphatase	+	-	-	-	-	ND	ND	ND
C4 esterase	-	ND	w	w	w	ND	ND	ND
C8 esterase lipase	-	ND	w	w	-	ND	ND	ND
C14 lipase	-	ND	-	-	-	-	ND	ND
Leucine arylamidase	+	-	-	-	-	ND	ND	ND
Valine arylamidase	-	ND	-	-	-	ND	ND	ND
Cystine arylamidase	-	ND	-	-	-	ND	ND	ND
Trypsin	-	ND	-	-	-	ND	ND	ND
α-Chymotrypsin	-	ND	-	-	-	ND	ND	ND
Acid phosphatase	+	ND	+	+	w	ND	ND	ND
Naphthol-AS-BI-phosphohydrolase	+	ND	w	w	w	ND	ND	ND
α-Galactosidase	+	+	+	+	+	ND	ND	ND
β-Galactosidase	+	+	+	+	-	ND	ND	ND
β-Glucuronidase	-	+	+	-	-	ND	ND	ND
α-Glucosidase	+	+	+	w	+	ND	ND	ND
β-Glucosidase	+	+	+	-	-	ND	ND	ND
N-Acetyl-β-glucosaminidase	+	-	-	-	-	ND	ND	ND
α-Mannosidase	-	ND	-	-	-	ND	ND	ND
α-Fucosidase	-	-	+	-	-	ND	ND	ND