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. 2021 Mar 19;13(6):1410. doi: 10.3390/cancers13061410

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Incubation of vulvar carcinoma cells with L-161,982. (a) 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromid (MTT) viability assay of A-431 cells after inhibition with different concentrations of L-161,982 for 72 h. * p = 0.001. (b) 5-Bromo-2’-Deoxyuridine (BrdU) proliferation assay of SW-954 calls after inhibition with different concentrations of L-161,982 for 72 h. * p = 0,001. (c,d) Western blot analysis of EP4 expression in A-431 and SW-954 cell lines and of benign epidermal tissue. (a,b) Error bars represent 95% confidence intervals. Wilcoxon test was used for the evaluation of viability/proliferation levels between vehicle and antagonist groups.