Skip to main content
. 2021 Mar 19;10(3):679. doi: 10.3390/cells10030679

Figure 3.

Figure 3

SRSF9 targets downstream exon to regulate AS of Caspase-2. (A)-(Upper) Schematic representation of D-10 minigene. Deleted sequences in the minigene are shown. D-10 was produced based on the deletion mutant of D9-2. (Middle) RT-PCR analysis of D-10 minigene in untreated, empty vector treated and SRSF9 expression plasmids treated HeLa cells. (Lower) Statistical differences are shown. (B)-(Upper) Sequences of wild type, M1 and M2 minigenes are shown.(Middle) RT-PCR analysis of M1 and M2 minigenes untreated, empty vector treated and SRSF9 expression plasmids treated HeLa cells are shown. (Lower) Statistical differences of RT-PCR analysis are shown. (C)-(Upper) Sequences of biotin-labeled oligonucleotides (wild type, M1 and M2) are shown. (Lower) Results of RNA-pulldown and immunoblotting for wild type, M1 and M2 with anti-SRSF9 and anti-SRSF3 antibodies are shown.