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. 2021 Mar 19;13(6):1408. doi: 10.3390/cancers13061408

Figure 4.

Figure 4

miR-29 targets constitute a vulnerability of melanoma. (A) expression of the nine putative miR-29 target genes upon miR-29 reactivation in miR-29 sponge melanoma cells; (B,C) expression of the nine putative miR-29 target genes upon miR-29 inhibitor or mimic transfection in miR-29 sponge melanoma cells (B) or human A375 melanoma cells (C). The mean ± SEM of one representative out of three independent experiments performed in triplicates is shown. Gene expression levels are normalized to β-Actin (mouse) or GAPDH (human); (D) validation of the efficacy of the ON-TARGETplus siRNA pool for the nine identified putative miR-29 targets; (E) effect of silencing the nine putative miR-29 targets on proliferation of A375 cells; (F) effect of silencing the nine putative miR-29 targets on focus formation of A375 cells; (G) quantification of colony formation shown in (F). The mean ± SEM of one representative out of two independent experiments performed in quadruplicates is shown. ns, not significant; * p < 0.05; ** p < 0.01; *** p < 0.001.