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. 2021 Mar 19;10(3):683. doi: 10.3390/cells10030683

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Biochemical pathway and metabolite analyses showing discrimination between Brachypodium metabolomes from different climatic regions of Turkey. FIE-HRMS derived metabolites which exhibited significant (one-way ANOVA correcting for false discovery rates, FDR) differences between accessions from different Turkish regions [6] were assessed using (A) metabolite set enrichment analysis (MSEA) or (B) the mummichog algorithm where significantly enriched pathways are also ranged for biological impact. (C) Exemplar metabolites exhibiting significant differences between accessions from different regions are shown using box and whisker plots. Regions 1a (red), 1c (yellow), 2 (purple), 3 (blue) and 4 (green) are indicated with Bd21 (dark orange) as the reference accession.