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. 2021 Mar 19;10(3):683. doi: 10.3390/cells10030683

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The metabolomes of Brachypodium accessions under drought stress and in controls. Eight-week-old Brachypodium accessions were transferred to the National Plant Phenomics Centre, Aberystwyth, UK. The watering was regulated to achieve three different soil water contents (SWC); 15%, 40% and 75% (control, CON). After 12 days of drought, leaves were sampled and the metabolomes were assessed using FIE-HRMS. (A) Discriminant Function Analysis of the derived metabolite profiles. Accessions are classified according of Turkish climate region of origin [6]: 1a (blue symbols), 1c (red symbols), 2 (black symbols), 3 (green symbols) and 4 (turquoise symbols). The 75% and 50% symbols are highlighted with a grey ellipsis to highlight their similarity but has no mathematical relevance. (B) Metabolomes from control (40%, 75% SWC) and droughted (15% SWC Brachypodium accessions forming the central (region 1a, 1c) and coastal (region 2, 3, 4) subpopulations were assessed for variation. Functionally important metabolites in drought responses; proline and sucrose are shown.