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. 2021 Mar 19;10(3):683. doi: 10.3390/cells10030683

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Key variables in the metabolomes of droughted Brachypodium accessions collected from different Turkish regions. Eight-week-old Brachypodium accessions were transferred to the National Plant Phenomics Centre, Aberystwyth, UK. The watering was regulated to achieve three different soil water contents (SWC): 15%, (droughted, DRO) 40% and 75% (control, CON). After 12 days of drought, leaves were sampled and the metabolomes were assessed using FIE-HRMS. The key variables discriminating between the droughted samples were identified by one way ANOVA (corrected for false discovery rates, FDR). (A) The relative levels of the m/z are displayed using a heatmap. Some mass-ions (m/z) were identified from comparison with databases and are labelled with names. Where m/z could not be identified these are given as the raw data values. (B) Box and whisker plot of exemplar metabolites which are increased in the central subpopulation (Turkish climatic region 1a, 1c) [6].