Table 1.
Sequence and mass spectra of the DNA- oligonucleotides.
| Name | Sequence (5′-3′) | MW Calc | MW Exp |
|---|---|---|---|
| TG4T | TGGGGT | 1861.3 | 1860.0 |
| TG6T | TGGGGGGT | 2519.4 | 2517.1 |
| T8 | TTTTTTTT | 2369.4 | 2364.4 |
| FdU5-T8 | FdU-FdU-FdU-FdU-FdU-TTTTTTTT | 3909.5 | 3912.5 |
| 1 FdU5-TG4T | FdU-FdU-FdU-FdU-FdU-TGGGGT | 3401.4 | 3399.2 |
| FdU5-TG6T | FdU-FdU-FdU-FdU-FdU-TGGGGGGT | 4059.5 | 4063.8 |
| FdU10-TG6T | (FdU)10-TGGGGGGT | 5599.6 | 5601.4 |
| 2 FAM-TG4T | FAM-TGGGGT | 2399.5 | 3 n.d. |
| FAM-TG6T | FAM-TGGGGGGT | 3057.57 | 3053.9 |
| FAM-T8 | FAM-TTTTTTTT | 2907.6 | 2902.8 |
| FAM-FdU5-T8 | FAM-FdU-FdU-FdU-FdU-FdU-TTTTTTTT | 4447.6 | 4447.7 |
| FAM-FdU5-TG4T | FAM-FdU-FdU-FdU-FdU-FdU-TGGGGT | 3971.6 | 3983.4 |
| FAM-FdU5-TG6T | FAM-FdU-FdU-FdU-FdU-FdU-TGGGGGGT | 4629.7 | 4685.1 |
1 FdU: 5-fluoro-2′-deoxyuridine, 2 FAM: fluorescein fluorophore, 3 n.d.: not determined.