Table 1.
MS (mass spectrometry) analysis of the over-expressed proteins from PEF-treated P. putida F1, compared to the nontreated bacteria.
| Protein IDs | Protein Name | Gene Name | Mol. Weight [kDa] | log2 * LFQ Intensity Nontreated | log2 * LFQ Intensity PEF-Treated |
|---|---|---|---|---|---|
| A5W5H2 | Alkyl hydroperoxide reductase/Thiol specific antioxidant/Mal allergen | Pput_3256 | 20.507 | 28.96 ± 0.43 | 33.33 ± 0.33 |
| A5VXD9 | TonB-dependent siderophore receptor | Pput_0376 | 88.459 | 19.18 ± 1.94 | 24.63 ± 1.18 |
| A5W124 | TonB-dependent siderophore receptor | Pput_1678 | 90.426 | ND | 24.12 ± 0.28 |
| A5W341 | TonB-dependent siderophore receptor | Pput_2412 | 79.564 | ND | 21.21 ± 0.73 |
| A5VZF9 | Integral membrane sensor signal transduction histidine kinase | Pput_1108 | 70.283 | ND | 21.84 ± 1.71 |
| A5VWS0 | Probable proton/glutamate-aspartate symporter | gltP | 47.566 | 21.96 ± 0.55 | 25.61 ± 0.18 |
| A5VXU7 | OmpW family protein | Pput_0539 | 24.19 | ND | 26.06 ± 1.47 |
| A5W1U8 | ABC-type metal ion transport system periplasmic component/surface adhesin-like protein | Pput_1965 | 30.162 | 18.25 ± 2.17 | 22.68 ± 0.16 |
| A5W1V5 | Propeptide, PepSY and peptidase M4 | Pput_1972 | 44.145 | ND | 24.35 ± 1.01 |
| A5VZG2 | L-glutamate ABC transporter membrane protein/L-aspartate ABC transporter membrane protein | Pput_1111 | 27.45 | 17.90 ± 1.57 | 22.14 ± 1.10 |
| A5W312 | Deoxyribonuclease I | Pput_2383 | 35.477 | ND | 21.05 ± 0.18 |
| A5WA94 | Choline/carnitine/betaine transporter | Pput_4934 | 73.501 | 20.91 ± 0.14 | 24.61 ± 0.52 |
Note: * Since a t-test must be done on normally distributed data, thus mass-spectrometry data is log-normalized, we applied log2 transformation to the (label-free quantitation) LFQ intensities.