. 2021 Mar 19;7(3):228. doi: 10.3390/jof7030228

Table 1.

Characteristics of the DNA extraction methods. The DNA extract measured by Nanodrop is expressed as a mean concentration (±standard deviation) C: chemical; M: mechanical; E: enzymatic; LB: NucliSENS^TM easyMAG^TM lysis buffer (BioMérieux, Marcy-l’Etoile, France); CB; ceramic beads; Ly: lyticase; PK: proteinase K; BALF: broncho-alveolar lavage fluid, DNA: deoxyribonucleic acid; RNA: ribonucleic acid.

Method	Kit	Company	Automate	Validated Sample	Validated Material	Nucleic Acids	Pretreatment			Sample Volume	Expected Elution Volume	Observed Elution Volume	Elution Appearance	DNA Quantification (ng/µL)
Method	Kit	Company	Automate	Validated Sample	Validated Material	Nucleic Acids	C	M	E	Sample Volume	Expected Elution Volume	Observed Elution Volume	Elution Appearance	DNA Quantification (ng/µL)
1	NucliSENS^TM EasyMAG^TM	BioMérieux	NucliSENS EasyMAG	Human	Whole blood, serum, plasma, stools, respiratory samples and other body fluids.	DNA/RNA	LB	CB	-	200 µL	110 µL	110 µL	Clear	27.0 (±6.6)
2	EZ1^TM DNA Blood 200 µL Kit	Qiagen	EZ1	Human	Whole blood	DNA	-	-	-	200 µL	100 µL	100 µL	Clear	30.5 (±5.5)
3	EZ1^TM DNA Blood 200 µL Kit + pretreatment	Qiagen	EZ1	Human	Whole blood	DNA	LB	CB	-	200 µL	100 µL	100 µL	Clear	10.4 (±2.7)
4	EZ1^TM DNA Tissue Kit + pretreatment	Qiagen	EZ1	Human	Whole dried blood, tissue, buccal cells, cultured cells, Paraffin-Embedded Tissue	DNA	-	-	Ly	200 µL	100 µL	100 µL	Clear	30.3 (±3.3)
5	QIAamp 96 DNA QIAcube HT Kit	Qiagen	QIAcube	Human	Whole blood, tissue, cells	DNA	-	-	PK	200 µL	120 µL	120 µL	Red tinted	30.2 (±6.6)
6	Macherey-Nagel™ Pathogène NucleoMag™	Fisher Scientific	KingFisher	Human	Whole blood, serum, plasma; tissue (e.g., ear notches); feces; swab wash solution	DNA/RNA	-	-	PK	150 µL	80 µL	80 µL	Clear	30.2 (±7.9)
7	Mag-Bind^TM Viral DNA/RNA	Omega Bio-tek	KingFisher	Human	Whole blood, serum, plasma, saliva, and other body fluids.	DNA/RNA	-	-	PK	200 µL	100 µL	100 µL	Clear	84.5 (±29.0)
8	MagMAX™ Viral/Pathogen Nucleic Acid Isolation Kit	Applied Biosystems	MGISP-960	Human	Whole blood, swabs, urine, and viral transport media	DNA/RNA	-	-	PK	400 µL	100 µL	100 µL	Red tinted	32.2 (±35.3)
9	Chemagic Viral DNA/RNA 300 kit H96	PerkinElmer	Chemagic 360	Human	Serum, plasma, saliva, nasal or oral swab, BALF	DNA/RNA	-	-	PK	300 µL	100 µL	100 µL	Red tinted	17.8 (±19.5)
10	Virus DNA/RNA Extraction kit	MGI	MGISP-960	Human	Serum, plasma, saliva, virus culture medium, throat swabs, BALF, sputum	DNA/RNA	-	-	PK	200 µL	100 µL	25 µL	Clear	61.3 (±42.6)
11	Bioextract^TM Superball^TM	Biosellal	KingFisher	Veterinary	Whole blood, milk, serum, organs	DNA/RNA	-	-	PK	200 µL	100 µL	100 µL	Clear	13.9 (±2.8)