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(a) Size change after extrusion through 100 nm membrane; (b) Volume loss after extrusion through 100 nm membrane (9 passages). All results are expressed as mean ± SD (n = 3). * means statistically significant differences (p < 0.05), n.s. means no statistically significant differences (p > 0.05) using one-way ANOVA followed by Tukey’s multiple comparison test.
